Prokaryotic vs. Eukaryotic Cells

Comparison of prokaryotic and eukaryotic cell structures

🦠 Prokaryotic vs. Eukaryotic Cells

Cell Theory

  1. All living things are composed of cells
  2. The cell is the basic unit of life
  3. All cells come from preexisting cells

Prokaryotic Cells

Characteristics:

  • No nucleus - DNA in nucleoid region
  • No membrane-bound organelles
  • Smaller (1-10 μm)
  • Examples: Bacteria, Archaea

Structures:

  • Nucleoid: region containing circular DNA
  • Ribosomes: protein synthesis (70S)
  • Cell wall: peptidoglycan (bacteria)
  • Plasma membrane: phospholipid bilayer
  • Capsule: protective outer layer (some)
  • Flagella: movement (some)
  • Pili: attachment, DNA transfer (some)

Eukaryotic Cells

Characteristics:

  • Nucleus with nuclear envelope
  • Membrane-bound organelles
  • Larger (10-100 μm)
  • Examples: Animals, plants, fungi, protists

Structures:

  • Nucleus: contains DNA
  • Membrane-bound organelles:
    • Mitochondria
    • Endoplasmic reticulum
    • Golgi apparatus
    • Lysosomes (animals)
    • Peroxisomes
  • Ribosomes: 80S (larger than prokaryotic)
  • Cytoskeleton: structural support, movement

Plant vs. Animal Cells

Unique to Plants:

  • Cell wall: cellulose
  • Chloroplasts: photosynthesis
  • Central vacuole: storage, turgor pressure
  • Plasmodesmata: channels between cells

Unique to Animals:

  • Centrioles: organize spindle fibers
  • Lysosomes: digestion (rare in plants)

Surface Area to Volume Ratio

Importance:

  • Limits cell size
  • Larger cells have lower SA:V ratio
  • Less efficient diffusion as cells grow
  • Cells divide to maintain high SA:V

Calculation: For a cube with side length a:

  • Surface area = 6a²
  • Volume = a³
  • Ratio = 6a²/a³ = 6/a

As a increases, ratio decreases!

Key Concepts

  1. Prokaryotes lack nucleus and organelles
  2. Eukaryotes have nucleus and organelles
  3. Plant cells have cell wall, chloroplasts, central vacuole
  4. Surface area to volume ratio limits cell size
  5. All cells have plasma membrane, ribosomes, DNA

📚 Practice Problems

1Problem 1easy

Question:

Create a comparison table of prokaryotic and eukaryotic cells, addressing: (a) presence of membrane-bound organelles, (b) DNA organization, (c) cell size, and (d) examples of organisms in each category.

💡 Show Solution

Prokaryotic vs Eukaryotic Cells:

| Feature | Prokaryotic | Eukaryotic | |---------|-------------|------------| | Nucleus | No true nucleus | Membrane-bound nucleus | | DNA organization | Circular DNA in nucleoid region | Linear DNA in chromosomes, wrapped around histones | | Membrane-bound organelles | Absent | Present (mitochondria, ER, Golgi, etc.) | | Ribosomes | 70S (smaller) | 80S (larger) in cytoplasm; 70S in mitochondria/chloroplasts | | Cell size | 0.1-5.0 μm (smaller) | 10-100 μm (larger) | | Cell division | Binary fission | Mitosis/meiosis | | Cytoskeleton | Simple (some have) | Complex (microtubules, microfilaments) | | Cell wall | Peptidoglycan (bacteria) | Cellulose (plants), chitin (fungi), or absent (animals) |

(a) Membrane-bound organelles:

Prokaryotic: ✗ No compartmentalization

  • All reactions occur in cytoplasm
  • Plasma membrane folds may increase surface area (mesosomes)

Eukaryotic: ✓ Extensive compartmentalization

  • Nucleus, mitochondria, ER, Golgi, lysosomes, peroxisomes
  • Allows specialized functions in separate compartments

(b) DNA organization:

Prokaryotic:

  • Single circular chromosome in nucleoid
  • No histones (except Archaea)
  • Plasmids (small circular DNA) common
  • Genes lack introns (continuous coding)

Eukaryotic:

  • Multiple linear chromosomes
  • DNA wrapped around histone proteins → chromatin
  • Genes contain introns and exons
  • Much more DNA (genome size 1000x larger typically)

(c) Cell size:

Prokaryotic: 0.1-5.0 μm

  • E. coli: ~2 μm long
  • Small size = high surface area to volume ratio
  • Efficient nutrient uptake

Eukaryotic: 10-100 μm

  • Human cell: ~10-30 μm
  • Larger, more complex
  • Require internal membrane systems

(d) Examples:

Prokaryotic:

  • Bacteria: E. coli, Streptococcus, Cyanobacteria
  • Archaea: Methanogens, Halophiles, Thermophiles

Eukaryotic:

  • Protists: Amoeba, Paramecium, Euglena
  • Fungi: Yeast, mushrooms
  • Plants: All plants
  • Animals: All animals

Prokaryotes: simple, no nucleus; Eukaryotes: complex, membrane-bound organelles\boxed{\text{Prokaryotes: simple, no nucleus; Eukaryotes: complex, membrane-bound organelles}}

2Problem 2easy

Question:

Create a comparison table of prokaryotic and eukaryotic cells, addressing: (a) presence of membrane-bound organelles, (b) DNA organization, (c) cell size, and (d) examples of organisms in each category.

💡 Show Solution

Prokaryotic vs Eukaryotic Cells:

| Feature | Prokaryotic | Eukaryotic | |---------|-------------|------------| | Nucleus | No true nucleus | Membrane-bound nucleus | | DNA organization | Circular DNA in nucleoid region | Linear DNA in chromosomes, wrapped around histones | | Membrane-bound organelles | Absent | Present (mitochondria, ER, Golgi, etc.) | | Ribosomes | 70S (smaller) | 80S (larger) in cytoplasm; 70S in mitochondria/chloroplasts | | Cell size | 0.1-5.0 μm (smaller) | 10-100 μm (larger) | | Cell division | Binary fission | Mitosis/meiosis | | Cytoskeleton | Simple (some have) | Complex (microtubules, microfilaments) | | Cell wall | Peptidoglycan (bacteria) | Cellulose (plants), chitin (fungi), or absent (animals) |

(a) Membrane-bound organelles:

Prokaryotic: ✗ No compartmentalization

  • All reactions occur in cytoplasm
  • Plasma membrane folds may increase surface area (mesosomes)

Eukaryotic: ✓ Extensive compartmentalization

  • Nucleus, mitochondria, ER, Golgi, lysosomes, peroxisomes
  • Allows specialized functions in separate compartments

(b) DNA organization:

Prokaryotic:

  • Single circular chromosome in nucleoid
  • No histones (except Archaea)
  • Plasmids (small circular DNA) common
  • Genes lack introns (continuous coding)

Eukaryotic:

  • Multiple linear chromosomes
  • DNA wrapped around histone proteins → chromatin
  • Genes contain introns and exons
  • Much more DNA (genome size 1000x larger typically)

(c) Cell size:

Prokaryotic: 0.1-5.0 μm

  • E. coli: ~2 μm long
  • Small size = high surface area to volume ratio
  • Efficient nutrient uptake

Eukaryotic: 10-100 μm

  • Human cell: ~10-30 μm
  • Larger, more complex
  • Require internal membrane systems

(d) Examples:

Prokaryotic:

  • Bacteria: E. coli, Streptococcus, Cyanobacteria
  • Archaea: Methanogens, Halophiles, Thermophiles

Eukaryotic:

  • Protists: Amoeba, Paramecium, Euglena
  • Fungi: Yeast, mushrooms
  • Plants: All plants
  • Animals: All animals

Prokaryotes: simple, no nucleus; Eukaryotes: complex, membrane-bound organelles\boxed{\text{Prokaryotes: simple, no nucleus; Eukaryotes: complex, membrane-bound organelles}}

3Problem 3easy

Question:

Create a comparison table of prokaryotic and eukaryotic cells, addressing: (a) presence of membrane-bound organelles, (b) DNA organization, (c) cell size, and (d) examples of organisms in each category.

💡 Show Solution

Prokaryotic vs Eukaryotic Cells:

| Feature | Prokaryotic | Eukaryotic | |---------|-------------|------------| | Nucleus | No true nucleus | Membrane-bound nucleus | | DNA organization | Circular DNA in nucleoid region | Linear DNA in chromosomes, wrapped around histones | | Membrane-bound organelles | Absent | Present (mitochondria, ER, Golgi, etc.) | | Ribosomes | 70S (smaller) | 80S (larger) in cytoplasm; 70S in mitochondria/chloroplasts | | Cell size | 0.1-5.0 μm (smaller) | 10-100 μm (larger) | | Cell division | Binary fission | Mitosis/meiosis | | Cytoskeleton | Simple (some have) | Complex (microtubules, microfilaments) | | Cell wall | Peptidoglycan (bacteria) | Cellulose (plants), chitin (fungi), or absent (animals) |

(a) Membrane-bound organelles:

Prokaryotic: ✗ No compartmentalization

  • All reactions occur in cytoplasm
  • Plasma membrane folds may increase surface area (mesosomes)

Eukaryotic: ✓ Extensive compartmentalization

  • Nucleus, mitochondria, ER, Golgi, lysosomes, peroxisomes
  • Allows specialized functions in separate compartments

(b) DNA organization:

Prokaryotic:

  • Single circular chromosome in nucleoid
  • No histones (except Archaea)
  • Plasmids (small circular DNA) common
  • Genes lack introns (continuous coding)

Eukaryotic:

  • Multiple linear chromosomes
  • DNA wrapped around histone proteins → chromatin
  • Genes contain introns and exons
  • Much more DNA (genome size 1000x larger typically)

(c) Cell size:

Prokaryotic: 0.1-5.0 μm

  • E. coli: ~2 μm long
  • Small size = high surface area to volume ratio
  • Efficient nutrient uptake

Eukaryotic: 10-100 μm

  • Human cell: ~10-30 μm
  • Larger, more complex
  • Require internal membrane systems

(d) Examples:

Prokaryotic:

  • Bacteria: E. coli, Streptococcus, Cyanobacteria
  • Archaea: Methanogens, Halophiles, Thermophiles

Eukaryotic:

  • Protists: Amoeba, Paramecium, Euglena
  • Fungi: Yeast, mushrooms
  • Plants: All plants
  • Animals: All animals

Prokaryotes: simple, no nucleus; Eukaryotes: complex, membrane-bound organelles\boxed{\text{Prokaryotes: simple, no nucleus; Eukaryotes: complex, membrane-bound organelles}}